Protein A Sepharose purification of Antibodies

• This protocol is useful for purifying antibodies from hybridoma cultures and serum.

Immunization protocol for rabbits

• This protocol is used for generating polyclonal antibodies to peptide ligands in rabbits.
• Citation: Novakowski KE, et al.A naturally occurring transcript variant of MARCO reveals the SRCR domain is critical for function. Immunol Cell Biol. 2016 Aug;94(7):646-55.

How to titrate antibodies for DIY ELISAs

• It’s often cheaper to buy the components of an ELISA (i.e. standard, capture & detecting antibodies) to make your own ELISA than to buy commercial ones. Here is an easy to follow protocol on how to systematically determine if you are using the right concentrations of antibodies.