“Blood CD33+HLA-DR- myeloid-derived suppressor cells are increased with age and a history of cancer” 2013. Verschoor et al. JLB

MDSCs (myeloid derived suppressor cells) are a recently discovered and very heterogenous cell type that appear to directly suppress T cell responses and their presence in the tumour or the circulation is an extremely poor prognostic marker.

The Bowdish lab recently dipped our foot into the confusing world of MDSCs because we were curious to know whether their frequency increases with age and if so, could this explain why age is a risk factor for many cancers. They do increase with age and this may explain why some adaptive immune responses decline with age. One thing we were very surprised to find is that individuals who had any history of cancer but were believed to be in remission had higher levels of these cells in circulation. This begs the question – did these people have higher levels and to begin with and is this why they were predisposed to cancer? Or do they still have subclinical tumours that promote the development of MDSCs? Or does the suppressive environment of the cancer microenvironment stay with you for life? More studies (not by us, that was enough) are warranted.

For the .pdf click here

For the supplementary data, click here.

Bowdish Lab Hosts Future Female Scientists (Canadian Association of Girls in Science).

On Thursday March 7th, the Bowdish Lab welcomed a group of five to twelve year old girls for a night for a night of science fun! In association with The Canadian Association for Girls In Science (CAGIS), the Bowdish lab opened its doors to promote and encourage our future young scientists.

CAGIS is an award winning national science club for girls aged 7-16, the purpose of which is to promote, educate and support interest and confidence in science, technology, engineering and mathematics among girls. Needless to say, The Bowdish Lab jumped at an opportunity to inspire such an eager group.

The night began with an introductory presentation by Dr. Bowdish which introduced theDawn gives out cards girls to basic concepts of immunology, good and bad germs and the members of the Bowdish Lab. Dr. Bowdish proposed a challenge to the girls; to encourage scientific inquiry, whoever asks the greatest number of scientific questions by the end of the night will win a prize! Every time a student asked a question, they would receive an official Dr. Dawn Bowdish business card to keep track. Dr. Bowdish nearly ran out of business cards IMG_0796 - Copybefore the end of the presentation!

 

The girls were then split into groups of two to three to take part in a variety of hands-on scientific activities. Of course, if the girls wanted to participate in awesome science activities, they need the proper scientific attire.

 

 

 

 

 

Girl & microscope

 

Members of the Bowdish lab organized five stations to help explain what we do as scientists. . Mike Dorrington introduced the girls to using a microscope to analyze anything from blood to nasal wash samples. Mike showed the girls how we separate blood into different components in order to isolate white blood cells. The girls were then able to observe the various types of white blood cells using pre-prepared slides. Bowdish lab organized five stations to help explain what we do as

 

Alicja & student

Alicja Puchta demonstrated how scientists develop special film to analyze anything from X-ray results to protein analysis. The girls were taken into a totally different world – the dark room. Special red lights allow for scientists to avoid exposing sensitive film to normal light. The girls were able to expose film to a variety of objects such as CD’s and pens and develop the film for their own take-home souvenir!

Avee Naidoo and Keith Lee introduced the concepts of acid and base chemistry using aColoured bubbles red-cabbage based pH indicator. The girls were allowed to use common household products to visualize changes in pH. Acids like vinegar turned the purple solution a bright neon pink, whereas bases such as baking soda turned the solution to blue and green. The grand finale was to add a piece of dry ice into a (green) basic solution. What would happen? The dry ice was able to turn the solution a variety of colors from green to blue to purple to pink in an eye catching manner.

Kyle Novakowski explained the concepts of dry ice and how we as scientists rely on it to send samples around the world while keeping them very cold. Students were shown what happens when dry ice is added to warm water and the process of sublimation. The resulting CO2 gas was then bubbled through a soapy solution to make spooky “boo bubbles” which the girls were able to hold in their very own hands. The girls were very excited to pop the bubbles and witness the escaping gas.

C02 is fun

Although the girls did not wish to be whisked away from the exciting applic

Pretty plates

ation s of dry ice, Dr. Preethi Jayanth was able to maintain the same level of fun at her stations. Our future scientist guests were able to observe various types of agar used to grow

After a few hours of exciting science, our champion question-asker was awarded a prize during a wrap-up Q&A session with Dr. Bowdish and the lab members. The immense amount of positive feedback and enthusiasm made for a wonderful night that we hope to experience again soon.bacteria. While peach and chocolate agar may sound appetizing – nobody would want to eat this stuff! The girls were given the opportunity to detect microbes on their own hands before and after washing! Two to three days later, the girls were sent pictures of all the bacteria on their hands which had grown on the agar.

Scientists plus girls excited about science

Bowdish Lab Cleans Up at the 53rd Annual Bay Area Science and Engineering Fair (BASEF)!

On Thursday May 21st, Bowdish Lab members were not only working hard as judges at the 53rd Annual BASEF fair, but also actively participating in presenting our research. Our high school students Jason Fan, winner of the IIDR summer studentship in 2012, and Leonard Rivet, a high school co-op student, both created fantastic posters and engaged judges in discussing their projects. Jason Fan (Right) received a Gold Merit Award and $100 for his poster entitled “Saving the World — One Ligand at a Time:  Novel Streptococcus pneumoniae Ligands for Macrophage Receptors”. Leonard Rivet (Left) received a Silver Merit Award and $80 for his poster entitled “Expression and Post-Translational Trimerization of MARCO in E.coli”.

Bowdish lab members Leonard Rivet & Jason Fan present their award winning posters at BASEF 2013.

Bowdish lab members Leonard Rivet & Jason Fan present their award winning posters at BASEF 2013.

Jason went on to win a silver award at the Canada wide Science Fair and to be accepted to McMaster’s prestigious Bachelor of Health Sciences program.

Congratulations to both our fantastic young scientists!

 

Bowdish Lab Attends 53rd Annual BASEF Science Fair

Dr. Bowdish and members of the lab attended the 53rd annual Bay Area Science and Engineering Fair (BASEF) as merit and special award judges on Thursday March 21st. All members of the lab were blown away by the sheer volume of young scientists and the incredible effort these students put into their projects. Bowdish Lab members judged projects focused on Health & Life sciences. Dr. Bowdish and Dr.Mossman judged for the IIDR Summer studentship, a 6-week summer internship in the IIDR, which is one of the most sought after prizes at the science fair.vWe are all looking forward to the 54th BASEF fair.

 

Eisha Ahmed (Abbey Park High School, Oakville) presents her award winning poster "Ara h Proteins, Peanuts, and Parasites". Eisha was the winner of the IIDR summer studentship and will be back at McMaster learning bioinformatics this summer.

Eisha Ahmed (Abbey Park High School, Oakville) presents her award winning poster “Ara h Proteins, Peanuts, and Parasites”. Eisha was the winner of the IIDR summer studentship and will be back at McMaster learning bioinformatics this summer.

 

Sarah Wu won second place for the IIDR summer studentship for her very impressive project developing a novel diagnostic for malaria.

Sarah Wu won second place for the IIDR summer studentship for her very impressive project developing a novel diagnostic for malaria.

 

Looking for an example of a teaching dossier or a candidate’s statement for tenure & promotion? See Dawn’s here.

I spoke at a post-doctoral fellow career day and there was a session on creating a teaching dossier. I am currently working on mine for tenure & promotion so if you’re curious here is my…

Candiate’s Statement

Teaching Philosophy & Dossier

…and here’s the documents that McMaster has prepared on preparing a teaching dossier and the formatting they prefer.

As a note, this is formatted in the way recommended by the Dept of Pathology & Molecular Medicine at McMaster. Other institutes & departments will have other stylistic preferences, if you’re able get an example from your department. I haven’t included my teaching tables (basically a summary of student ratings and attendance of my courses). They are not particularly interesting but if there is interest I am happy to post them.

Don’t be shy  – I’m a big fan of crowdsourcing so tell me what you think & what can be improved. Also, this is a teaching example of me as an assistant professor, I am happy to share my documents I created when I was applying for positions, just email & ask.

Changing of the undergraduate guards.

The changing of the guards (or at least undergrads) happened this summer in the Bowdish Lab. We wish our departing undergraduates Keith Lee (onwards to medical school), Samanthy Balachandran (onwards to graduate school) and Peter Mu (radical that he is – onward to getting a job!) all the best in their future endeavors!
The Bowdish Lab is excited to welcome our new undergraduate students Prasant Bharadwaj, a MITACS summer student from India, James Han, an iSci student, and Dessi Loukouv, a co-op student working collaboratively with Qu Biologics. Netusha Thevaranjan will be staying on as a summer/thesis student.
Peter Mu, Samanthy Balachandran and Keith Lee - the 2012/13 crop of undergrads as they move on to their next adventure.

Peter Mu, Samanthy Balachandran and Keith Lee – the 2012/13 crop of undergrads as they move on to their next adventure.

Nothing says "Thanks for all the hard work" like carrot cake.

Nothing says “Thanks for all the hard work” like carrot cake.

Dr. Dawn Bowdish receives a research award from the Ontario Lung Association and Pfizer Canada (and gets to attend a swanky event to receive it!)

Dawn was thrilled to attend the annual Breathe! gala event hosted as a fundraising event for the Ontario Lung Association to receive an award donated by Pfizer Canada and administered by the Ontario Lung association. Not only did it give her a chance to dress up and go out (a rare event!) for an evening of good food and drink but she was inspired to meet some of the spokespeople of the event, including Helene Campbell, who spent her time waiting for a double lung transplant starting an immensely successful social media campaign to increase organ donation, Ann Marie Cerato, a lung cancer survivor and Kayla Baker, a young sarcoidosis patient on a waiting list for a lung transplant. Not only was it inspiring to meet these brave, bold heroes but it was humbling to be in a room full of people whose lives were all touched by the research funded by the Ontario Lung Association.

Dawn standing beside the real heros of the event, Helene Campbell, double lung transplant recipient and idiopathic fibrosis patient, Ann Marie Cerato, lung cancer survivor and Kayla Baker sarcoidosis patient waiting for a lung transplant.

So what did Dawn win her award for? You can take a look at the YouTube video here or read on…

President of Pfizer Canada, John Helou (L) and Dr. John Granton, chair elect of the Ontario Lung Association (R) present Dr. Dawn Bowdish with the OLA-Pfizer Research Award.

Description of research funded by the Ontario Lung Association-Pfizer Ca award

neumonia is the sixth most common cause of death in Canada.  The incidence of pneumonia rises steeply in individuals over the age of 65 years and approximately 90% of deaths due to pneumonia occur in the elderly (>65 yrs).  Current prevention strategies are inadequate as the vaccination does not prevent pneumonia in most elderly individuals. Recent research from the Bowdish lab demonstrates that one of the reasons the elderly are so susceptible to pneumonia is that their immune systems cannot control the bacteria that normally live in our sinuses. The immune systems of healthy adults can keep the bacteria (Streptococcus pneumoniae) in the sinuses (“pneumococcal carriage”) and eventually clear them, but for reasons we don’t understand, the immune systems of the elderly cannot and as a result the bacteria break through the immune barriers of the sinuses and spread to the lungs, which results in pneumonia.

Although most people think that the elderly get sick because their immune systems “just don’t work”, in fact our data demonstrate that they recruit more white blood cells to the sinuses when they encounter the bacteria that cause pneumonia than healthy adults. Even though they have more white blood cells in the sinuses they don’t seem to be as good at recognizing and killing bacteria. Our goal is to figure out why they have overactive recruitment of white blood cells and why they aren’t as good at killing bacteria as white blood cells from healthy adults.

We have developed what we believe to be the world’s only Aged mouse model of colonization by pneumonia causing bacteria. This allows us to study how the immune system responds to the presence of bacteria in the sinuses in real-time. In addition we have a bank of white blood cells from adults and elderly patients that allow us to confirm the importance of our mouse studies in people.

Hospitalizations and deaths due to pneumonia are unacceptably high in the elderly. This is likely because vaccination of the elderly only does not protect against pneumonia. New methods for preventing infection are urgently required. Our recent data demonstrates that containment and clearance of pneumococcal carriage is impaired in age and results in increased susceptibility to pneumonia; however the mechanisms by which immune control of the sinuses fails remain to be discovered. Prevention of colonization of the sinuses will therefore be essential for control of pneumonia in this population. In order to develop novel therapeutic interventions for the elderly it will be necessary to discover the mechanisms by which bacterial recognition, killing are impaired in the sinuses.

Helene Campbell, conceivably, the most charming woman alive and the recipient of a double lung transplant, leads a dance with the Hon Deborah Mathews (MPP). The music failed so I held my iPod up to the Mike and we played Metric's "Sympathy".