The novel object recognition test is used to evaluate recognition memory in mice. The test consists of two sessions in the open field arena, separated by an intersession interval (ISI). In the first session, the Training, the mouse is free to explore and familiarize itself with two identical objects; during the second session, Testing, one of the now-familiar objects is replaced by a novel object. A mouse that remembers the familiar object will spend more time exploring the novel one. By using a short ISI (3 hours), this test assesses short-term recognition memory.
The Open Field test measures anxiety, willingness to explore, and general mobility and behaviour.