Fiona Whelan and Ryan Buensuceso, sit down with the IIDR’s Dr. Dawn Bowdish to discuss how and why she chose science as a career.
Hear the podcast here.
Twitter handles:
Fiona Whelan (@whelanfj)
Ryan Buensuceso (@rcbuensuceso)
Dr. Dawn Bowdish (@MsMacrophage)
Michael G. DeGroote Institute for Infectious Disease Research (IIDR) (@McMasterIIDR)
Running a research lab is expensive (very expensive!) and the vast majority of funding to the Bowdish lab comes from taxpayers by way of agencies like the Canadian Institutes for Health Research and the National Science and Engineering Council or from generous donations to non-profit agencies like the Ontario Lung Association. We do get donations from individuals on occasion and these are even more special to us because they mean that our work has inspired someone to donate their hard-earned dollars. Below is a particularly heart-warming story of a 5 year old who donated $5 of her allowance to help us keep all the grandmas and grandpas healthy. The video below shows what we bought with that money and allows us to express our thanks.
Your donations, no matter how big or small, are special to us. They strengthen our resolve to work harder and smarter to make research breakthroughs, the inspire us to communicate our research with you, the public, and they remind us of that we are working for you, the public.
If you are inspired to donate money for research to improve lung health we’d encourage you to consider donating to the Ontario Lung Association’s Breathing as One campaign.
The McMaster Immunology Research Centre is fundraising with the Lung Association to create a new PhD scholarship in lung health. If you would like to donate to that, please contact Dr Bowdish at bowdish@mcmaster.ca or Dr. Carl Richards (director)richards@mcmaster.ca .
And lastly, if you’ve been inspired by the work of the Bowdish lab and would like to make a donation, thank you! Please contact Dr. Bowdish (bowdish@mcmaster.ca, 905-525-9140 x22313)
Thank you to all our donors. Thank you for your generosity and your belief in our work!
Who’s got the strongest lab at McMaster? I do! We’ve had a great run of success at the Bowdish lab with fully all of our students receiving prestigious scholarships! And that’s not to mention our other successes such as our summer scholarship winners (Jason Fan – IIDR), our students who got their degree and moved on to great things (Dr. Fan Fei – manager of Mass Spec facility, Dr. Mike Dorrington – PDF at the NIH), and our PDF, Dr. Chris Verschoor who got a faculty position!
Bowdish lab scholarship winners 2015/2016: Netusha Thevaranjan (CIHR-PhD), Avee Naidoo (CIHR-PhD), Kyle Novakowski (NSERC-PhD), Pat Schenck (CIHR-PhD), Dessi Loukov (CIHR-PhD), Justin Boyle (NSERC-MSC).
The Bowdish lab has been working with the Ontario Lung Association to encourage politicians to support Bill #41 – The Lung Health Act. You may not be aware that of all the common chronic diseases (e.g. cardiovascular, diabetes), lung disease is the only one that doesn’t have a dedicated action plan. Because of this rates of lung disease are higher then they need to be and many “best practices” for treating or preventing lung disease are not in place, compromising the health of many Ontarians and increasing health care costs. For this reason MPP Kathryn McGarry put forth a private members bill to create a Lung Health Act for Ontario. Bill 41 would entail establishing a Lung Health Advisory Council to make recommendations to the Minister of Health and Long-Term Care on lung health issues; and requiring the Minister to develop and implement an Ontario Lung Health Action Plan respecting research, prevention, diagnosis and treatment of lung disease. In Novemver our lab attended a provincial lobby day where we discussed the importance of this bill with politicians. On June 6th Dawn gave a deputation to the Committee on Social Policy on why this Bill would be of particular importance to older adults.
To see the entire 4 hours of deputations (ouch!) click here.
To read more of the FAQ around the Lung Health Act click here.
To read more about the Ontario Lung Association’s draft Lung Health Action Plan, click here. (Bonus – you can donate to the Lung Association to support this work via that link too!).
Tammy Villeneuve (OLA), Andrea Kellner, Justin Boyle, MPP Dave Levac, Kyle Novakowski, Dessi Loukov and Dr. Dawn Bowdish meet to discuss the Lung Health Act.
The Bowdish lab attends lobby day in November 2015 to discuss the importance of the Lung Health Act with MPPs.
Walter Gretzky supports the Lung Health Act – you should too! (with Justin Boyle & Dessi Loukov)
Dawn at Queen’s Park after her deputation on Bill 41- The Lung Health Act. The Bowdish lab participates in democracy!
PhD student Dessi Loukov in the lab of Dr. Dawn Bowdish, recently published a study showing that splenomegaly in old mice is a result of extramedullary hematopoiesis, and that this increased monopoiesis is driven by age-associated increase in TNF. The study compared changes in the microarchitecture and composition of the spleen in old and young mice and found that in old mice, there was an increase in the size and cellularity of the red pulp (the site of hematopoiesis of myeloid precursors). To study the role of TNF in the development of extramedullary hematopoiesis, they used TNF KO mice and found that these mice did not have increased extramedullary monopoiesis. Furthermore, they demonstrated that increased splenic myelopoiesis was a result of the aging microenvironment. This work suggests that strategies which aim to decrease the inflammatory microenvironment that comes with aging, would be effective in reducing inflammatory diseases propagated by cells of the myeloid lineage. Read More
Congratulations to Kyle for passing his comprehensive exam and moving from PhD student to PhD candidate! Well done.
Kyle, getting twice as much work done in the tissue culture hood.
Colonization of Streptococcus pneumoniae within the upper respiratory tract (URT) of elderly individuals is a major concern, as it often results in the development of pneumonia, which can be deadly in this population. A study published by MIRC Masters’ student Netusha Thevaranjan, under the supervision of Dr. Dawn Bowdish, examined how
aging can change the composition of the respiratory microbial community and consequently, impact bacterial colonization. Using a mouse model of pneumococcal colonization, the study characterized the composition of the URT microbiota in young, middle-aged, and old mice in both the naïve state, and throughout the course of nasopharyngeal colonization with S. pneumoniae. It was shown that the composition of the URT microbiota differs with age, and that colonization with S. pneumoniae in older mice disrupted pre-existing microbial communities.
Furthermore, the study demonstrated that there were several interspecies interactions between S. pneumoniae and resident microbes. In particular,Streptococcus interacted competitively with Staphylococcus and synergistically with Haemophilus. This work provides insight into how aging influences bacterial colonization, and understanding the relationship between these two factors can help create strategies to protect the elderly from age-associated infections and disease. Read More
See our “Protocol” page for more cell culture protocols.
Culturing, Maintaining and Seeding the nasopharyngeal cell line Detroit 562
Macrophages play a critical role in innate immunity by detecting, engulfing and destroying pathogenic bacteria and alerting neighbouring immune cells to join the fight against infection. They have many different receptors on their cell surface that allow them to carry out these important processes. A particular group of receptors called Scavenger Receptors are vital to this response. A recent study published in Immunology and Cell Biology by PhD student Kyle Novakowski from the laboratory of Dr. Dawn Bowdish has uncovered a mechanism by which a specific scavenger receptor contributes to macrophage-specific antibacterial immunity.
Scavenger Receptors are evolutionarily ancient and have evolved to recognize a wide array of pathogens by detecting ligands that are common across many pathogenic organisms. A particularly important Scavenger Receptor is Macrophage Receptor with Collagenous Structure, or MARCO. MARCO has been shown to significantly contribute to the clearance of Streptococcus pneumoniae colonization of the nose and in models of pneumococcal pneumonia. The NSERC-funded study took a unique approach to functionally characterizing how MARCO contributes to innate immunity by studying a naturally-occurring variant of the receptor. The study highlighted the importance of a particular domain of the receptor that is required for macrophages to bind and internalize ligands. The study also showed that the domain is necessary to enhance the pro-inflammatory response to pathogenic Streptococcus pneumoniae and can enhance cellular adhesion; both vital to proper macrophage functions.
To read the article, please click here.
Inflammation causes white blood cells to drastically change shape. This cell is viewed at 8000x! #MacArtofResearch pic.twitter.com/lbWllnnDK6
— MacArtofResearch (@McArtofResearch) April 2, 2016