General

Cytokine ELISA 

• This is a general protocol for performing ELISAs.

How to titrate antibodies for DIY ELISAs

• It’s often cheaper to buy the components of an ELISA (i.e. standard, capture & detecting antibodies) to make your own ELISA than to buy commercial ones. Here is an easy to follow protocol on how to systematically determine if you are using the right concentrations of antibodies.

Using the Ella SimplePlex System to measure cytokines

Specific ELISAs

Human C-reactive protein (CRP)

• Citation: Verschoor CP, et al.DNA methylation patterns are related to co-morbidity status and circulating C-reactive protein levels in the nursing home elderly.Exp Gerontol. 2018 May;105:47-52. doi: 10.1016/j.exger.2017.10.010