Cloning

 Site-directed mutagenesis

Immunoprecipitation

• This protocol demonstrates how to immunoprecipitate a protein from a cell lysate.

Co-immunoprecipitation

Transfection of HEK293T cells with PEI

• This is a cheap and effective protocol for transfecting HEK293T cells using PEI.  Note: In Jan 2013 we posted an updated version (version 2) with clarifications requested by users. We have another variant here. We have adapted this protocol for 24 well plates. To see the adapted version click Protocol for 24-Well Plates