This protocol is used to extract the RNA from tissue or a cell sample using TRIzol and also includes recommendations for 260/280 and 260/230 values when measuring the concentration of extracted RNA using a nanodrop spectrometer.
- This protocol is for isolating RNA from cell lines or tissues for RNA sequencing.
RNA preparation for RNAseq (RNA sequencing/transcriptomics)
- The protocol details the steps required fro processing (eukaryote) RNA for RNA sequencing experiments including ribosomal RNA depletion, quality control and quantification, cDNA preparation and labelling with adaptors.